Fig. 2. In situ production of NO throughout preimplantation development detected using DAF-FM. (a) zygote, (b) two-cell, (c) four-cell, (d) eight-cell, (e) morula, (f) blastocyst, (g) in vivo-derived mouse blastocyst. PB, polar body; ICM, inner cell mass; TE, trophectoderm, Bars, 20 µm.