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Figure 5


Fig. 5. Relative fluorescence of DAF-FM in the area selected for bleaching. Two-cell embryos stained with DAF-FM with or without Mitotracker Deep Red were subjected to photobleaching using the 633nm laser line and DAF-FM fluorescence quantified using laser-scanning confocal microscopy. Values are means ± s.e.m. and are corrected for acquisitional bleach and normalised for comparison. The number of determinations is shown in parentheses.