Fig. 4. TJ ultrastructure and strand number are not altered by tMLCK expression. (A,B) Representative images of TJ from monolayers not induced (A) or induced (B) to express tMLCK. Magnification, 62,500x. (C) The number of strands in each TJ replica was counted at 160 nm intervals over a total distance of 195 µm and 185 µm in monolayers induced (open circles) or not induced (closed circles) to express tMLCK, respectively. This resulted in 56 and 54 strand counts in induced and uninduced monolayers, respectively. The histogram shows the frequency with which each strand number was observed. There was no significant difference between induced and uninduced monolayers.