Fig. 6. Overexpression of a dominant-negative mutant of aPKC enhances redistribution of gp135 and suppresses phenotype of mLgl knockdown. (A) mLgl kd cells (24-15) or control cells (21-1) on permeable filters were infected with adenovirus expression vectors encoding LacZ, wild-type aPKC (aPKC wt) or dominant-negative aPKC (aPKC dn) for 2 hours. The cells were then incubated with LC medium for 18 hours. (A) Cells were fixed and double stained with anti-aPKC and anti-gp135 antibodies. Notice that the overexpression of aPKC dn but not aPKC wt in the control or the mLgl kd cells decreases the peripheral gp135 staining in depolarized cells. (B) Quantification of the cells with cell peripheral gp135 staining in A. Each photograph represents the projected views of confocal sections. Bars, 20 µm.