Fig. 5. T. gondii activates ERK1/2 and PKB independently of MyD88, unlike LPS. MØs from wild-type (WT) and MyD88 knockout (KO) mice were incubated with parasites (6:1 ratio of tachyzoites to cells, >90% infected) or LPS (100 ng/ml), and at the indicated time points, samples were collected for western blot analysis measuring total and phosphorylated forms of each signaling intermediate. This experiment was repeated 4 times with the same result.