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Figure 4


Fig. 4. Renal clearance of tracers within the proximal pronephric duct is restricted to the megalin/LRP2 expression domain. Wild-type embryos (72 hpf) were injected with 70kDa-FD (green) and renal clearance of tracer into the pronephric duct tubular cells evaluated 1.5 hours later on whole mounts (A,C) or transversal sections (D,F) by confocal fluorescence microscopy. (B,C) Immunostaining for megalin/LRP2 (red) demonstrates that renal clearance of 70kDa-FD occurs within the expression domain of the receptor. (E,F) Transversal sections show apical localization of megalin/LRP2. Bars, 100 µm (A-C) and 5 µm (D-F).