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Figure 3


Fig. 3. Immunohistochemical staining reveals that the knocked-in {alpha}3 connexin restores targeting of {alpha}8-G22R mutant subunits to gap junctions in the lens fiber cells. (A,B) {alpha}8 connexin staining (red) in cross sections of (A) {alpha}3(-/-) {alpha}8(G22R/-) and (B) {alpha}3(-/-) {alpha}8(G22R/KI{alpha}3) lenses of 3-week-old mice. (C,D) Merged images of {alpha}8 connexin (red) and F-actin (FITC-phalloidin staining, green) in cross sections of (C) {alpha}3(-/-) {alpha}8(G22R/-) and (D) {alpha}3(-/-) {alpha}8(G22R/KI{alpha}3) lenses. Boxed regions in C and D are magnified in C' and D', respectively, showing that very few punctate fluorescent spots appear in the peripheral fiber cells of the {alpha}3(-/-) {alpha}8(G22R/-) lens, whereas typical punctate fluorescent signals of gap junctions predominantly localize in the long sides of the fiber cells of the {alpha}3(-/-) {alpha}8(G22R/KI{alpha}3) lens. Bar, 20 µm.