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Figure 4


Fig. 4. Western blot data show that the {alpha}8-G22R connexin protein level is greatly increased in the lenses of {alpha}3(-/-) {alpha}8(G22R/KI{alpha}3) mice. Membrane-enriched lens protein samples were prepared from different mice aged 3 weeks. Lane 1, wild type {alpha}3(+/+) {alpha}8(+/+); lane 2, {alpha}3(-/-) {alpha}8(KI{alpha}3/-); lane 3, {alpha}3(-/-) {alpha}8(G22R/KI{alpha}3); lane 4, {alpha}3(-/-) {alpha}8(G22R/-). Equal amounts of lens samples were loaded onto the gel and probed with (A) anti-{alpha}3 connexin antibody or (B) anti-{alpha}8 connexin antibody. Lane 1 is the wild-type control showing the endogenous {alpha}3 and {alpha}8 connexins. All three mutant mice contain no endogenous wild-type {alpha}3 and {alpha}8 alleles, thus the bands in lanes 2 and 3 in A are knocked-in {alpha}3 connexin. The band in lane 3 in B is the {alpha}8-G22R mutant. The molecular mass markers are listed on the left and give values in kDa.