Fig. 3. Effect of long-term culture on the expression of CD117, CD34, Sca-1 and CD49e. Thymus-derived MSCs were analyzed by flow cytometry at passages 11 and 42. Whereas the level of expression of Sca-1 was around 2 log values (versus 1 log control), CD117 expression decreased to nearly control levels, CD34 expression was lost, and expression of CD49e increased 1 log value.