Fig. 5. GGS-MAPPIT analysis of CIS and SOCS2 interactions with the LR. (A,B) HEK293T cells (A) or TF-1 cells (B) were transiently co-transfected with plasmids encoding the chimeric bait constructs with the different LR motifs or with the FKBP12 control bait, and the pMG2-CIS, pMG2-SOCS2 prey constructs, combined with the pXP2d2-rPAP1-luci. The transfected cells were either stimulated for 24 hours with Epo or were left untreated (NS, not stimulated). Luciferase measurements were performed in triplicate. Data are expressed as mean fold induction (Epo stimulated/NS) + s.e.m. (C) FACS analysis shows the expression of the different chimeric GGS bait receptors in TF-1 cells. The grey filled curves represent the parental TF-1 cells; open lines, the transiently transfected TF-1 cells.