Fig. 3. Localisation of vibrator protein in spermatocytes. An antibody raised against vibrator recombinant protein was used in immunolocalisation studies. Microtubules are shown in green, vibrator in red (also shown in monochrome), DNA is in blue. (A) Vibrator localises to membranous structures in primary spermatocytes including the nuclear and plasma membranes. (B) At anaphase, vibrator decorates the region occupied by the central spindle microtubules. This region contains many membranous structures including mitochondria. (C) At the onion stage of early spermatid development vibrator associates with the mitochondrial derivative (nebenkern). Bars, 10 µm.