Fig. 7. Loss of vibrator protein affects furrow stability. (A) DIC images and (B) selected corresponding frames from time-lapse imaging of a vib^S110416 / Df(3R)Dl-BX12 mutant primary spermatocyte expressing GFP-tagged ß-tubulin. Time (in minutes) is shown relative to anaphase onset (0). In this cell, furrow initiation occurred 12 minutes after anaphase onset. Although the central spindle microtubules form correctly they fail to become fully compressed (38 minutes). The cell remains in this state until 51 minutes after anaphase onset when there is a rapid dissociation of the cleavage-furrow membrane from the central spindle region within 1 minute (notice the disappearance of the membrane at 52 minutes, arrow). Dissolution of the parafusorial membranes does not occur in this cell (38-62 minutes). See supplementary material, Movie 2. Bar, 10 µm.