Fig. 4. Time-lapse analysis of the dynamic MORN1 localization during T. gondii cell division. (A) MORN1-YFP (green) and H2B-mRFP (red, nucleus) transgenic parasites were imaged every 10 minutes. The entire dataset is available as Movie 3 in the supplementary material. Distances between the daughter and mother cell MORN1 structures (B) are plotted over time (C, average of both daughters given). (D-K) Selected time points (as indicated in C) are shown at higher magnification for one cell (boxed in A). Arrow in (D) highlights centrocone, double arrows the first sign of daughter buds. Centrally forming apical ring is marked by arrow before (G) and after extrusion (H). An additional weakly labeled MORN1 is apparent in I, arrow. Note ring constriction from J to K.