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Figure 1


Fig. 1. Immunoblot detection of Cx40 and Cx43 expression in untransfected and stably transfected HeLa and HEK cells. Whole-cell lysates (10 µg protein for HeLa cells, 20 µg protein for HEK cells in Fig. 1A; 10 µg protein for all samples in Fig. 1B) were resolved by SDS-PAGE, transferred to membranes and blotted with (A) anti-Cx40 or (B) anti-Cx43 antibodies. Migration of molecular weight markers is indicated to the left of the blots.