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Figure 5


Fig. 5. Spermine blockade of gap junctional currents in N2a cells expressing wild-type (wt) or mutant Cx40. 2 mM spermine was applied unilaterally (in one pipet) during recording of a family of junctional currents. (A) Currents between a pair of cells (stably) expressing wtCx40 were blocked when Vj was positive (center). (B) Currents between a pair of cells (transiently) expressing Cx40E12S,E13G were not affected by 2 mM spermine. (C) Currents between a pair of cells co-expressing wtCx40 (stably) and Cx40E12S,E13G (transiently) were not affected by 2 mM spermine. (D) Currents between a pair of cells co-expressing wtCx40 (stably) and Cx43D12S,K13G (transiently) were slightly affected by 2 mM spermine.