Fig. 6. Single gap junction channel events detected in whole-cell two-current recordings obtained from N2a cells expressing different connexins (during a 30-second Vj pulse of -40 mV): wild-type Cx40 (stably, top trace), wild-type Cx43 (stably, second trace), Cx40E12S,E13G (transiently, third trace), wild-type Cx43 (stably) co-expressed with wild-type Cx40 (transiently, fourth trace), and wild-type Cx43 (stably) co-expressed with Cx40E12S,E13G (transiently, fifth trace). Panels A and B show channel events on different time scales. Panel B shows a 2-seconds excerpt (indicated with a bar above each trace in panel A) displayed at an expanded time scale to illustrate single channel events more clearly.