Fig. 4. Calcofluor staining of cellulose in transformed protoplasts. (A) single protoplasts transformed with WAK1-GFP were stained with 1 µg/ml calcofluor and the cellulose (calcofluor) or the GFP fluorescence was captured on a standard fluorescence microscope 1 hour or 3 days after transformation. Cells were incubated in RM. (B) Protoplasts were incubated in RM (0) or RM plus 2.5 µM isoxaben (+isox) for 3 days, and then stained with calcofluor and visualized by standard fluorescence microscopy for calcofluor. Red, chlorophyll; green, GFP; blue, calcofluor. Magnification, x300.