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Figure 1


Fig. 1. Subcellular distribution of HA-GLUT8 expressed in HeLa cells. Cells were grown on coverslips and either transfected with HA-GLUT8 or HA-GLUT8-LL/AA alone, or co-transfected with HA-GLUT8 and dominant-negative dynamin-K44A, or dynamin-K44A alone. After transfection (48 hours), non-permeabilized (-TX-100) and permeabilized (+TX-100) cells were stained for the HA-epitope tag and analyzed by confocal laser scanning microscopy. Bars, 20 µm.