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Figure 10


Fig. 10. Steady-state distribution of HA-GLUT8s within the endosomal/lysosomal pathway and the Golgi apparatus. HeLa cells were transfected with HA-GLUT8 and cultured for 3 days. Then, the cells were stained with either Bodipy-TR ceramide or LysoTracker-Red, or stained for AP-1 or AP-2 using a monoclonal antibody against {gamma}- or µ2-adaptin as described in the Materials and Methods. After co-staining for the HA-epitope tag, the cells and analyzed by confocal laser scanning microscopy. No difference in the staining pattern was observed for HA-GLUT8 and HA-GLUT8/YTRF (data not shown). Squares indicate magnified regions (mag.). Bars, 20 µm.