Fig. 4. RNAi-mediated knockdown of µ2-adaptin in HeLa cells. Cells were transfected with a plasmid construct for the µ2 siRNA (pSuper/µ2-1) or with empty pSuper vector (con). After 6 days in cell culture, the cells were harvested and lysed. Then, the proteins (20 µg/lane) were subjected to SDS-PAGE, and the expression of adaptins in the crude lysates was determined by western blotting using antibodies against -adaptin (112 kDa), ß-adaptin (106 kDa), -adaptin (104 kDa), µ2-adaptin (50 kDa), and -tubulin as described in the Materials and Methods.