Fig. 6. Localization of JIL-1 and Chromator in mutant polytene chromosomes. (A) Triple labelings with the JIL-1 pAb Hope (green), the C-terminal Chromator mAb 6H11 (red) and Hoechst 33258 (blue) of polytene squashes from wild-type (upper panel), Chro⁷¹/Chro⁶¹² (middle panel), and JIL-1^z2/JIL-1^z2 (lower panel) female third-instar larvae. The composite image (comp) is shown to the left. The mAb 6H11 epitope is not present in either of the truncated Chro⁷¹ or Chro⁶¹² proteins. (B) Double labeling with the N-terminal Chromator mAb 12H9 (red) and Hoechst 33258 (blue) of polytene squashes from a Chro⁷¹/Chro⁶¹² female third-instar larvae. The composite image (comp) is shown to the left. (C) Labeling with the JIL-1 pAb Hope of polytene squashes from wild-type (left micrograph) and Chro⁷¹/Chro⁶¹² (right micrograph) male third-instar larvae. Note the upregulation of JIL-1 on the male X chromosome (X) of both wild-type and Chro⁷¹/Chro⁶¹² mutant larvae.