Fig. 5. Calpain regulates spreading and adhesion of DCs. Using phase-contrast video time-lapse microscopy, untreated and ALLM or ALLN treated cells were filmed for 240 minutes while spreading on poly-L-lysine coated dishes. (A) Area of cell spread on the substratum measured using motion analysis software. The graphs illustrate the mean percentage of cells ± s.e.m. from three experiments. The area of treated cells was significantly different to that in untreated cells (P<0.005) by ANOVA analysis. (B) Percentage of adhesion of DCs on poly-L-lysine-coated wells 30 minutes after plating. The graphs illustrate the mean percentage of cells ± s.e.m. from three experiments ^*P<0.05 compared with untreated cells (Student's t-test). ALLM and ALLN treatments resulted in decreased area of spread and adhesion efficiency.