Fig. 8. Calpain cleaves the podosome proteins WASP, Pyk2 and talin. Total lysates of untreated and ALLM- or ALLN-treated plated DCs were separated by SDS-PAGE and western blotted. Immunoblotting with the corresponding antibodies showed that WASP, Pyk2 and talin but not vinculin or Src were cleaved by calpain. Two calpain cleavage products of WASP of 40 kDa and a 45 kDa (asterisks) were detected. One cleavage product of Pyk2 (50 kDa, asterisk) and three of talin (70 kDa, 90 kDa and 190 kDa, asterisks) were detected.