Fig. 2. Mapping the RyR2-interacting region of snapin. (A) Schematic diagram of snapin sub-overlapping fragments. (B) Protein expression of snapin fragments in yeast: protein extracts (50 µg per sample) of yeast Y190 transformed with plasmids as indicated were analysed by western blotting using Ab^HA (12% SDS-PAGE gel; pACT2: empty vector). (C) Yeast two-hybrid liquid ß-galactosidase assay of yeast Y190 transformed with the plasmids as indicated. Mean values of ß-galactosidase units obtained from five individual colonies per sample, with normalisation against the positive control, are shown.