Fig. 6. Effect of snapin on [³H]ryanodine binding to skeletal muscle RyR. Skeletal muscle heavy SR (50 µg) was incubated at a range of buffered free Ca²⁺ concentrations with 10 nM [³H]ryanodine in the presence or absence of 1 µM GST-NSnp. Non-specific binding was determined by the addition of 10 µM unlabelled ryanodine. A mean value of specific binding was determined from at least three separate experiments. For each Ca²⁺ concentration, the left column in the pair is the control and the right column is in the presence of snapin.