Fig. 6. Tsg101 is found in Hrs-containing microdomains. HEp-2 cells were transfected with Rab5^Q79L and stained for immunofluorescence confocal microscopy with anti-Tsg101 and anti-EEA1 or anti-Hrs antibodies. (A) Two selected enlarged endosomes. Yellow indicates colocalisation. (B) Level of colocalisation of Tsg101 and EEA1 or Hrs on membranes of Rab5^Q79L-containing endosomes, quantified as described in Materials and Methods. Error bars give the mean + s.e.m. EEA1, n=16; Hrs, n=19, P=0.008.