Fig. 8. Induction of DE-cadherin expression by Dsh correlates with low levels of cytosolic Arm. cl8Dshmyc cells were incubated without Cu²⁺ or in the presence of Cu²⁺ for 24 hours and triple stained with antibodies against Arm, DE-cadherin and Dsh (Dsh image not shown). In the absence of Cu²⁺, Arm and DE-cadherin were barely detectable by immunofluorescence (left panel). After 24 hours of Dsh overexpression, strong Arm staining was detected in the cytoplasm and nuclei of many cells, whereas other cells showed only weak staining for Arm (right panel, top), despite expression of high levels of Dsh (not shown). Staining for DE-cadherin (right panel, middle) was strong at cell-cell contacts in cells expressing low levels of cytoplasmic Arm and low in cells expressing high levels of cytoplasmic Arm (right panel, bottom). Note that Arm staining in nuclei had approximately the same intensity as cytoplasmic staining, resulting in uniform staining of cells (see Orsulic and Peifer, 1996). Bar, 10 µm.