Fig. 1. Sdc1 mediates cell spreading in B82L fibroblasts. B82L cells were plated on nitrocellulose coated with 60 µg/ml non-immune mouse IgG (A,B), 60 µg/ml mAb 281.2 (C,D) or 200 µg/ml S4ED pAb (E,F). Fetal bovine serum (FBS) (10%) was added to cells plated in B, D and F. Cells were incubated for 2 hours then fixed with paraformaldehyde and stained with Rhodamine-conjugated phalloidin. Bar, 50 µm.