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Figure 2


Fig. 2. Expression of p53 and Mdm2 in HCMV-infected HUVECs. (A) Protein levels were semi-quantified using western blot analysis of the total cellular protein extracts from HCMV-infected HUVECs (MOI=1.0) during the first 13 dpi. Dynamic changes in protein levels of the total p53, HCMV-immediate-early (IE) proteins (68/72 kDa) and Mdm2 were shown. (B,C) Graphs showing the corresponding mRNA and protein levels. The nucleus and cytoplasm bars show the proportional changes between the two subcellular compartments during the course of infection, based on IFA. All values have been normalized against corresponding actin level, and represent mean (± s.e.m.) of three different batches of HCMV-infected HUVECs (MOI=1.0). Each experiment was conducted in triplicate.