Fig. 7. The time-dependent changes in ubiquitinated p53 pattern in HCMV-infected HUVECs. Western blot with anti-p53 (fl-393) polyclonal antibody was employed to demonstrate the ubiquitinated p53 pattern in uninfected (0 dpi) and HCMV-infected (1-11 dpi) HUVECs with (B) or without (A) 10 µM MG132 (proteasome inhibitor) treatment. In the MG132-treated cells, mono-ubiquitinated p53 (p53-Ub₍₁₎ at 60 kDa) significantly increased towards the later stages of infection (3 dpi onwards). The increased levels were observed also for the corresponding double mono-ubiquitinated bands (p53-Ub₍₂₎ at 75 kDa). By contrast, the multiple mono-ubiquitinated p53 bands (p53-Ub_(n)) of higher molecular masses tended to decrease towards the later stages of infection (3 dpi onwards). Molecular masses are given on the left in kDa.