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Figure 3


Fig. 3. Characterization of AQP2 in CnA{alpha} +/+ and -/- mice. Expression of total AQP2 was visualized by immunohistochemistry in kidney sections of (A) +/+ and (B) -/- mice using the DAB chromagen and counterstaining with hematoxylin. Data are representative of at least four mice. (C) AQP2 expression was determined by western blotting with specific antibodies. Characteristic forms of glycosylated protein (Gly) is observed at 35-45 kDa and non-glycosylated (NG) at 29 kDa. Lanes 1-3, +/+; lanes 4-6, +/-; lanes 7-9, -/-. Expression of actin served as a control. Results from two independent experiments were evaluated by semi-quantitative analysis and results are shown as bar graphs as the mean ± s.e.m.