Fig. 8. Effect of CsA on DDAVP-mediated regulation of AQP2. (A) Expression of p-AQP2 and total AQP2 was determined in whole kidney lysates of control, DDAVP, and DDAVP + CsA-treated mice by immunoblotting with specific antibodies. Actin was included as a control. Lanes 1-3, control; lanes 4-6, DDAVP; lanes 7-9, CsA + DDAVP. Amounts of total and p-AQP2 were evaluated by semi-quantitative analysis and results are shown in bar graphs. (B) AQP2 localization was detected by immunohistochemistry in kidney IM sections with the DAB chromagen and sections were counterstained with hematoxylin. (i) Control, (ii) DDAVP, (iii) DDAVP + CsA. Shown are typical data of three mice per group. Magnification is 40x Results of immunohistochemistry were evaluated by semi-quantitative analysis and are shown in as the mean ± s.e.m. ^*P<0.05.