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Figure 9


Fig. 9. AtMND1 and AHP2 interact in a yeast two-hybrid (Y2H) assay. Yeast strain AH109 was transformed with the indicated plasmids and grown on synthetic drop-out (SD) media lacking the amino acids leucine and tryptophan (SD -Leu/-Trp) or leucine, tryptophan and histidine (SD -Leu/-Trp/-His). Only the combination of plasmids containing AtMND1 fused to the GAL4-DNA binding domain and AHP2 fused to the GAL4 activator domain (pGBKT7/AtMND1, pGAD10/AHP2) or vice versa (pGBKT7/AHP2, pGAD424/AtMND1) enabled strain AH109 to grow on SD -Leu/-Trp/-His, demonstrating an interaction between AtMND1 and AHP2.