Fig. 2. Induction of pro-survival signals in co-cultures of mpcs with MPs. (A) Examples of immunoblots of Bcl-2, phosphorylated Akt (Akt-P) and phosphorylated ERK1/2 (ERK1/2-P) in myoblasts (Mb), myotubes (MT), MP cultures and co-cultures. Detection of ß-actin was used to check the protein amount deposited in each well. (B) Apoptosis of mpcs was detected with annexin V (white bars) and DIOC-6 (black bars) in co-cultures of mpcs with MPs in the presence of H₂O₂ at low concentration (0.2 mM) or cytochalasin D (1 µg/ml). Results are means ± s.e.m. of three experiments.