Fig. 5. In the giant body, protein signals are round shaped. Either they have a pale central core (PML, BLM, TOPOIII, SP100, DAXX, ATRX) or they are homogeneously stained (HP1ß). These different patterns can be observed by decomposition into colours and enlargement (x2.8) of the protein signals colocalising in the giant body. (A) Triple immunostaining of an ICF nucleus showing that the DAXX layer (red) is larger than the ATRX layer (blue), which in turn is larger than the HP1ß layer (green). (B-D) Triple immunostaining showing respectively that BLM, TOPOIII and SP100 layers (red) are located between PML (green) and DAXX (blue) layers.