Fig. 6. Growth of yeast carrying vha55 mutants. (A-B) 1x10⁶ cells were taken from mid-log phase culture, serially diluted 1:5 eight times, and each dilution spotted in a grid on both pH 5.0 (permissive, left panels), and pH 7.5 (restrictive, right panels) selective medium plates, and grown at 30°C for 2-3 days. (A) Low-copy plasmid. Column 1, wild-type, no plasmid; 2, vma2, no plasmid; 3, vma2 with VMA2 rescue; 4, vma2 with vha55 rescue; 5, vma2 with vha55::GFP rescue; 6, vma2 with vha55 ORF::GFP rescue. (B) As A, except that vma2 yeast were carrying vha55::GFP cDNA in a low-copy plasmid, with point mutations corresponding to the vha55 mutant alleles. Column 1, vha55::GFP cDNA; 2, empty vector; 3, vha55^SzA1; 4, vha55^SzA9; 5, vha55^SzA9 (both changes); 6, vha55^SzA12; 7, vha55^7e1; 8, vha55¹⁴.