Fig. 1. Ferlin domains and FER-1 mutations. FER-1 and several homologs are aligned with their domain composition indicated. Most ferlins have four or more C2 domains, which are shown as colored shapes reflecting similar positions in the various ferlin proteins. DYSF (striped rectangle) has nested DysfN and DysfC domains; TM (black diamond) transmembrane domain. Predicted protein changes for all fer-1 alleles are shown here and described in Table 1; ^*, stop codon. Letter designations for C2 domains in dysferlin are from Davis et al. (Davis et al., 2000). Dotted lines below FER-1 indicate antigenic sites for anti-DysfNC (left) and anti-AZ10 (right). See supplementary material (Table S1 and Fig. S1) for additional homologs.