Fig. 5. Immunofluorescent localization of FER-1 in sperm. (A-J) Deconvoluted images of immunostaining performed on spermatids or TEA-activated spermatozoa. Spermatids were dissected from 2-3 day virginized him-5 or fer-1(hc47) males. Cells were stained with anti-AZ10 (green) antibodies to detect FER-1 and with 1CB4 (red) to label MOs. Bar, 2 µm. (A-D) FER-1 is localized to punctate structures and colocalizes with 1CB4. (E-H) FER-1 is localized in the plasma membrane of mature sperm, and partially colocalizes with 1CB4. Arrowheads indicate visible pseudopods. (I-J) fer-1(hc47) sperm, which have a stop codon prior to the AZ10 antigenic site and make no FER-1. (J) No staining is detected in fer-1(hc47) sperm at the same exposure used for normal sperm.