Fig. 4. Identification of the p75-binding domain in JPO2. (A) JPO2 (wt) or its deletion mutants (indicated) produced by in vitro transcription and translation were incubated with GST or GST-p75^347-471 pre-bound to GS beads in the presence of BSA. Eluted proteins fractionated by SDS-PAGE were visualized by autoradiography (top panels) or staining with Coomassie Blue (bottom panels). Migration positions of molecular mass markers, GST and GST-p75^347-471are indicated. None of the JPO2 deletions mutants bound to GST alone (data not shown). (B) Schematic representation of wild-type JPO2 and deletion mutants used in panel A. Predicted helices and ß strands are indicated below the full-length sequence as filled and hatched boxes, respectively. Binding affinities of deletion mutant proteins relative to wild-type protein are indicated (+, +/- or -).