Fig. 2. Effect of AZI on proliferation of non-transformed NIH-3T3 fibroblasts. (A) Western blot analysis of 50 µg of total cell lysate from NIH-3T3 cells stably infected with retroviral vectors pWZL or pWZL-AZI. (B) Proliferation of control () and AZI-overexpressing NIH-3T3 cells () grown in low serum (0.5% BCS). Cells were plated at a density of 5x10⁴ cells in 12-well plates and the total number of cells per well were counted. Error bars indicate s.e.m. (C) To assess saturation density, control () and AZI-overexpressing NIH-3T3 cells () were plated at 1x10⁴ cells in six-well plates in media containing 10% BCS and cell number assessed over 11 days.