Fig. 7. AZI shows ability to attenuate cyclin D1 degradation. Cyclin D1, wild-type AZI and AZI_117-140 were synthesized by in vitro transcription and translation in separate reactions. All three proteins were radiolabeled with [³⁵S]methionine. Translated proteins were combined and incubated in an ATP-regenerating buffer for 0, 30, 60, 120 and 180 minutes. The amount of cyclin D1 remaining at the indicated time points was assessed by SDS-PAGE and PhosphorImager analysis (lower panel). Results are representative of two independent experiments. , cyclin D1 alone; , Cyclin D1 + wild-type AZI; , % Cyclin D1 + AZI_117-140.