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Figure 8


Fig. 8. Interaction of AZI and cyclin D1 in vitro and effect of AZI{Delta}117-140 mutation. (A) 293 cells were transfected with combinations of plasmids expressing S-tagged AZI, FLAG-tagged antizyme and HA-tagged cyclin D1. After 48 hours, lysates were collected and incubated with S-protein agarose. AZI-interacting proteins were identified by western blot using anti-HA, anti-FLAG or anti-AZI antibodies. (B) Immunoprecipitation of transfected S-tagged AZI{Delta}117-140, FLAG-tagged antizyme and HA-tagged cyclin D1 as described above. AZI{Delta}117-140 interacted with cyclin D1 but not antizyme.