Fig. 1. Expression of TRPL-eGFP in photoreceptors of transgenic Drosophila. (A) Western blot analysis of protein extracts obtained from heads of wild-type flies (wt), TRPL-null mutant (yw; trpl³⁰²), and TRPL-eGFP-expressing flies in TRPL-null (yw; trpl³⁰²; trpl-eGFP) or wild-type background (yw; trpl-eGFP). The same blot was probed with antibodies directed against TRPL, TRP and GFP as indicated. Proteins obtained from three Drosophila heads were loaded per lane. (B) The green fluorescing deep pseudopupil of TRPL-eGFP-expressing flies (trpl-eGFP) raised in the dark (left panel). Fluorescence of the pseudopupil was not observed when the flies were raised in the light (middle panel), nor was it observed in wild-type eyes (right panel). (C) Subcellular localization of TRPL-eGFP in dark- and light-raised transgenic flies (yw; trpl-eGFP). eGFP-fluorescence was detected in intact eyes after optical neutralization of the cornea by water immersion. Flies were kept in the dark (left panel) or under orange light (right panel) for 16 hours. The insets show the central area of the eye at higher magnification. Scale bar, 15 µm.