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Figure 6


Fig. 6. Components of the phototransduction cascade are required for TRPL-eGFP translocation. Water immersion images of eGFP fluorescence in the eyes of the following mutants raised for 16 hours in the dark or in orange light are shown: (A,B) w; G{alpha}q1; trpl-eGFP, (C,D) w; G{alpha}q1/Df(2R)vg135; trpl-eGFP/+, (E,F) w norpAP24; trpl-eGFP/+, (G,H) norpAP57; bw/trpl-eGFP; st/+ (note: this mutant has red eyes, hence eGFP-fluorescence in the cell body was shielded by screening pigments), (I,J) yw; trpl-eGFP; trpP343, (K,L) yw; inaD1 cn bw; trpl-eGFP, (M,N) w inaFP106x; trpl-eGFP/+, (O,P) w; trpl-eGFP/+; trpP365/+. Bar, 15 µm.