Fig. 1. Suppression activity of ArfGAPs, BET1, BOS1, OLE1, and GYP6 for arf1-16 and arf1-17 ts mutants. (A) arf1-16 (NYY16-1) and arf1-17 (NYY17-1) cells were transformed with pNY24-GLO3 (2 µ GLO3), pNY24-GCS1 (2 µ GCS1), pNY24-AGE1 (2 µ AGE1), pNY24-AGE2 (2 µ AGE2), and the transformants were grown at 23°C. Cells were diluted to a final concentration of 1.0 at OD 600, and 10 µl of 10-fold dilutions were spotted on MCD (minimal glucose casamino acid medium) (-Trp) plates. The plates were incubated at the indicated temperatures for 2 days. Integrants of wild-type ARF1 (WT, NYY0-1) and mutants transformed with pYO324 (vector) were used as controls. (B) arf1-16 and arf1-17 cells were transformed with pNY25-BET1 (2 µ BET1), pNY25-BOS1 (2 µ BOS1), pNY25-OLE1 (2 µ OLE1), and pNY25-GYP6 (2 µ GYP6), and the transformants were spotted on MVD (-Leu) plates and grown as described in A. Integrants of wild-type ARF1 (WT) and mutants transformed with pYO325 (vector) were used as controls.