Fig. 2. DKK1 mRNA and protein expression is restricted to the stromal-vascular fraction of human adipose tissue. (A) DKK1 mRNA levels were measured using real-time RT PCR in subcutaneous and omental stromal-vascular cells and mature adipocytes from eight unrelated subjects. (B) Dkk1 protein expression in vivo. Western blot analysis of whole-cell lysates from subcutaneous stromal-vascular cells and mature adipocytes from four unrelated subjects. (C) DKK1 mRNA expression in human and mouse adipose tissues. Total RNA was extracted from stromal-vascular cells and mature adipocytes from four mice (pooled samples), confluent 3T3-L1 cells and one human subject, and RT-PCR for mouse and human DKK1 was performed. RNA isolated from whole mouse embryo and 3T3-L1 cells stably overexpressing human DKK1 were used as positive controls for mouse and human PCRs, respectively. Data were normalised using 18S control. Preads, preadipocytes; Ads, adipocytes; 3T3, 3T3-L1 cells; m, mouse; h, human; +, positive control; ^**P<0.01.