Fig. 3. Expression of DKK2 and DKK3 during adipogenesis. (A) Expression of Dkk2 and Dkk3 mRNA in mouse adipose tissue was determined using RNA obtained in Fig. 2C. (B) 3T3-L1 preadipocytes were induced to differentiate and total RNA extracted at the time points indicated. Dkk2 and Dkk3 mRNA levels were measured using real-time RT-PCR. Results are expressed as fold difference relative to the baseline (time 0). All results the mean ± s.e.m. of four independent experiments. (C) Human subcutaneous preadipocytes from six unrelated subjects were differentiated in vitro and total RNA extracted at the time points indicated. mRNA levels of DKK2 and DKK3 were determined by real-time RT-PCR. Results are expressed as fold difference relative to baseline (time 0). Preads, preadipocytes; Ads, adipocytes; 3T3, 3T3-L1 cells; m, mouse; h, human; +, positive control. ^*P<0.05, ^**P<0.01, ^***P<0.001.