Fig. 6. Expression of Dkk1 receptors is regulated during early human adipogenesis. Subcutaneous preadipocytes from eight unrelated subjects were differentiated in vitro and total RNA extracted at the time points indicated (see Table 1). mRNA levels of (A) KRM1 and (B) LRP5 and LRP6 were determined by real-time RT PCR. Results are expressed as fold difference relative to baseline (time 0). ^*P<0.05, ^***P<0.001.