Fig. 5. Double strand break repair (DSBR) protein, Rad50, is associated with UBF in cells infected with E4 deleted virus, dl366. HeLa cells were infected or mock-infected with the mutant virus and fixed at 18 hours post-infection. (A) An infected cell showing the location of Rad50 (in red), which is seen alongside DBP (in green). In the merged image, DAPI is included to illustrate the extent of the nucleus. (B) Two uninfected cells, with Rad50 in red and UBF in green. (C) An infected cell, labelled as in B. All images are of a single focal plane approximately 0.3 µm depth; bar, 10 µm.