Fig. 7. Antiserum to UBF retards viral DNA replication in permeabilised cells. (A) Viral DNA recovered from a number of replication assays, digested with KpnI and separated by electrophoresis and stained with ethidium bromide. The antiserum added to each assay is indicated above each lane, the size of the DIG-labelled molecular mass markers is indicated on the right hand side of the gel and each viral DNA fragment is labelled a-i on the left hand side of the gel. In addition, the DNA fragments corresponding to the left- and right-most ends of the viral DNA are marked with arrows. (B) The levels of DIG-labelled nucleotides incorporated into the DNA fragments. This was done by Southern transfer of the gel in A to nylon membrane and the DIG label detected as described in the Materials and Methods. (C) The same as in B but with a longer exposure. Below C is a schematic of the viral genome indicating where KpnI cuts the viral genome and the position of DNA fragments on the genome.